Gel+Electrophoresis

The ability of the Polycationic amphiphilic CDs to compact plasmid DNA can be examined by gel electrophoresis .Since the negatively charged plasmid DNA can be compacted inside and around the cationic vesicle formed by the amphiphilic CDs the hydrodynamic size would be smaller then that of free plasmid DNA. The smaller hydrodynamic size results in a lower mobility over the gel. Also if the DNA is protected by the CD derivative it is no longer accessible to the staining reagent.
 * Gel Electrophoresis **